Tool
Blood-Test Request Builder for Hair Loss
what blood tests should I ask for, and what is a good ferritin level for hair growth
Two things make a blood-test appointment go badly: not knowing what to ask for, and
being told a result is "normal" when the guides put it well below the level associated with hair growth.
many doctors order incomplete panels
DL02 Standard laboratory reference ranges are designed to identify disease, not optimize health.
DL02 So this builds the list, and then shows you the bands
the guides publish for the three numbers people actually ask about.
The list to ask for
Core panel 8
On the list whatever your symptoms are.
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Complete Blood Count (CBC)
Measures red blood cells, white blood cells, hemoglobin, and platelets.
DL02present in 25-30% of hair loss patients
DL02 -
Ferritin
Ferritin reflects your body’s iron reserves better than serum iron alone.
DL02affecting up to 40% of women with thinning hair
DL02 -
Serum iron with TIBC
Serum iron measures circulating iron; Total Iron Binding Capacity (TIBC) shows how much iron your blood could carry.
DL02 -
TSH
TSH is the screening test for thyroid function.
DL02Both hypothyroidism and hyperthyroidism disrupt the hair growth cycle, affecting roughly 15% of people with hair loss
DL02 -
Free T4
Free T4 provides additional information about active thyroid hormone.
DL02 -
Vitamin D (25-hydroxyvitamin D)
The 25-hydroxyvitamin D test [25(OH)D] is the only reliable way to assess vitamin D status.
DL07Vitamin D deficiency appears in approximately 80% of alopecia patients
DL02 -
Vitamin B12
B12 deficiency causes hair loss and is more common than many realize
DL02B12 · "Normal" Range 200-900 pg/mL · Optimal for Hair >400 pg/mL
W08 -
Zinc
Zinc is essential for hair follicle function and protein synthesis.
DL02Zinc · "Normal" Range 70-120 µg/dL · Optimal for Hair 80-100 µg/dL
W08
Full thyroid panel 1
Added because you ticked fatigue, cold intolerance or a weight change.
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Free T3
The most biologically active thyroid hormone—the one that actually produces effects in your cells, including hair follicles.
DL06
Thyroid antibodies 2
Added because you ticked thyroid-type symptoms or patchy loss.
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TPO antibodies
Identifies autoimmune thyroid disease (Hashimoto’s thyroiditis).
DL02Present in 90% of Hashimoto’s thyroiditis patients.
DL06 -
Thyroglobulin antibodies
Another marker of autoimmune thyroid disease, often tested alongside TPO antibodies for complete assessment.
DL02
Autoimmune screen 1
Added because you ticked patchy loss or a scaly, red scalp.
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ANA (antinuclear antibody)
A screening test for autoimmune conditions.
DL02Note that some healthy people have low-positive ANA without disease.
DL02
Hormone panel 7
Added because you ticked irregular periods, adult acne or excess facial/body hair.
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Total testosterone
Measures total circulating testosterone.
DL0275% of women with hormonal hair loss have normal total testosterone
DL02 -
Free testosterone
The biologically active form. Calculated from total testosterone and SHBG.
DL02Only 6.8% of women with female pattern hair loss had elevated free testosterone
W08 -
DHEA-S
Measures adrenal androgen production.
DL02 -
SHBG
SHBG binds testosterone, reducing its availability.
DL02 -
Androstenedione
An androgen precursor.
DL02Research shows 25% of women with signs of androgen excess have normal testosterone but elevated androstenedione.
DL02 -
Prolactin
Elevated prolactin (from pituitary issues or certain medications) can cause hair loss.
DL02 -
LH and FSH
These pituitary hormones regulate ovarian function.
DL02LH/FSH ratio: >2:1 suggests PCOS
W08
If PCOS is on the table 3
Added because irregular periods came with acne or excess hair.
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AMH (anti-Müllerian hormone)
AMH: Can replace ultrasound for diagnosis
W05 -
Fasting insulin and glucose
Fasting insulin and glucose: To assess insulin resistance
W05 -
17-hydroxyprogesterone
17-hydroxyprogesterone: To rule out congenital adrenal hyperplasia
W05
Fuller iron studies 1
Added because you ticked heavy or long periods.
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Transferrin saturation
Transferrin Saturation: Percentage of iron-carrying capacity being used
W08Ferritin is an acute-phase reactant, meaning it can be falsely elevated by inflammation, infection, or liver disease.
DL05
No hormone panel added
The guides do not route men to androgen testing by default: Male pattern baldness is caused by genetic sensitivity to normal testosterone levels, not elevated testosterone.
DL02
Timing, so the numbers mean something
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Ferritin and iron studies
Best: Fasting or at least 2 hours after eating
W08 -
Ferritin and iron studies
Iron supplements can falsely elevate; stop 24-48 hours before testing
W08 -
Ferritin and iron studies
Avoid: Testing during or right after menstruation (ferritin drops)
W08 -
Thyroid tests
Best: Morning, fasting
W08 -
Thyroid tests
Biotin supplements can interfere—stop 2-3 days before
W08 -
Thyroid tests
Stop biotin supplements at least 2 days before testing (some sources recommend 3-7 days)
DL06 -
Thyroid tests
If on thyroid medication, test before taking your morning dose
DL06 -
Hormone tests
Hormone testing in women should ideally occur on days 2-5 of the menstrual cycle
DL02 -
Hormone tests
Best: Day 2-5 of menstrual cycle (follicular phase)
W08 -
Hormone tests
Post-menopausal women can test any time.
DL02 -
Vitamin D
Any time, no special preparation
W08 -
Retesting
Retest ferritin after 8-12 weeks, vitamin D after 3 months, and thyroid markers after 6-12 weeks if on medication.
DL02
Hand this over
Bring a list: Know exactly which tests you want
W08
Already have results?
Type a number in and this shows which published band it falls in, and the sentence that
band comes from. That is all it does. It is not a reading of your health, and the bands are the guides’,
not your laboratory’s. Blood test interpretation requires clinical context.
W08
Enter a value to see which band the guide puts it in.
- Critical deficiency (<20 ng/mL) DL05
Hair loss very likely to be occurring or imminent.
DL05- Suboptimal (20-40 ng/mL) DL05
Hair loss probable or contributing factor.
DL05- Adequate for hair (40-60 ng/mL) DL05
Minimum level to support hair growth.
DL05- Good for hair (60-70 ng/mL) DL05
Supports healthy hair growth.
DL05- Optimal for hair (70-100 ng/mL) DL05
Ideal for hair density and growth.
DL05
Outside that range: Ferritin above 200 ng/mL without supplementation (may indicate iron overload or inflammation)
DL05
Enter a value to see which band the guide puts it in.
- Low TSH (below 0.4 mIU/L) DL02
Suggests hyperthyroidism.
DL02- Lab normal: 0.4-4.0 mIU/L DL06
AACE recommends: 0.3-3.0 mIU/L
DL06- Optimal for symptoms: 0.5-2.5 mIU/L DL06
AACE recommends: 0.3-3.0 mIU/L
DL06- Many people continue experiencing fatigue, cold intolerance, and hair loss when their TSH is technically "normal" (say, 3.5) but not optimal. DL06
Lab normal: 0.4-4.0 mIU/L
DL06- "High-normal" (3.5-4.5): May indicate subclinical hypothyroidism W08
Lab normal: 0.4-4.0 mIU/L
DL06- Elevated TSH (above 4.0 mIU/L) DL02
Even "subclinical" hypothyroidism (TSH 4-10 with normal T4) can cause hair thinning.
DL02
Enter a value to see which band the guide puts it in.
- <20 · Deficient · High risk of hair loss; requires correction DL07
Levels below 20 ng/mL significantly increase the risk of telogen effluvium
DL07- 20-29 · Insufficient · Suboptimal; may contribute to thinning DL07
Many people continue losing hair with levels in the 20-29 ng/mL range
DL07- 30-50 · Optimal · Best range for hair health and immune function DL07
Optimal vitamin D level for hair health is 30-50 ng/mL
DL07- 50-100 · Adequate · Safe; may not provide additional benefit DL07
The Endocrine Society Clinical Practice Guidelines (2011) identified 30-100 ng/mL as the desirable range, with 21-29 ng/mL considered insufficient and <20 ng/mL deficient.
DL07
Outside that range: >100 · Excessive · Risk of toxicity; avoid
DL07
Our own guides do not agree on the vitamin D target
Three of the drafts behind this tool give three different optimal ranges. Rather than pick one, here are all three with their sources:
- DL07
Optimal vitamin D level for hair health is 30-50 ng/mL
DL07 - W08
Optimal: 40-60 ng/mL
W08 - DL02
50-80 ng/mL is ideal for most people
DL02
The scale above uses DL07’s bands, because DL07 is the vitamin D guide. The disagreement is real and worth knowing about before you argue a number with anyone.
Context for the three numbers
- Ferritin
Laboratory "normal" ferritin (10-15 ng/mL) is too low for optimal hair growth
DL05Using 41 ng/mL as the cutoff yields 98% sensitivity and 98% specificity
DL05 - Ferritin
Research suggests best outcomes at ferritin levels of 70 ng/mL or higher.
DL05Women with telogen effluvium had mean ferritin of 14.7 ng/mL vs 43.5 ng/mL in controls
W08 - Ferritin
iron deficiency was the most prevalent etiology of female hair loss, accounting for 70.3% of cases
W08You can have iron deficiency hair loss WITHOUT anemia.
W08 - TSH
Many people continue experiencing fatigue, cold intolerance, and hair loss when their TSH is technically "normal" (say, 3.5) but not optimal.
DL0650% of people with hyperthyroidism and 33% with hypothyroidism experience hair loss
DL06 - TSH
Request TSH, Free T4, Free T3, TPO antibodies, and TG antibodies.
DL0617.3% of women with female pattern hair loss had elevated anti-TPO antibodies
W08 - Vitamin D
56% of people with androgenetic alopecia have vitamin D deficiency (vs 24% in controls)
DL07 - Hormones
PCOS affects 11-13% of women globally
W05This affects 60-80% of women with PCOS
W05
What the guides say not to bother with
Comprehensive metabolic panels, liver function, and kidney function are good to have but rarely directly explain hair loss.
DL02Genetic testing for hair loss is available but doesn’t change treatment decisions. Heavy metal testing is popular but rarely clinically useful.
DL02